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جداسازی، شناسایی و بهینهسازی کشت جدایههای بومی یاروویا لیپولیتیکا از نمونههای محیطی برای تولید اسید چرب غیراشباع | ||
| زیست شناسی میکروبی | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 24 شهریور 1405 | ||
| نوع مقاله: پژوهشی- انگلیسی | ||
| شناسه دیجیتال (DOI): 10.22108/bjm.2026.149541.1685 | ||
| نویسندگان | ||
| Morteza Nadeb1؛ soheil aghaee* 1؛ mohammad reza zolfaghary2؛ Mojgan Saghazadeh2؛ Tahereh komeili3 | ||
| 1Honarestan39,HN12 | ||
| 2هیت علمی دانشگاه ازاد قم | ||
| 3هیت علمی دانشگاه علوم پزشکی قم | ||
| چکیده | ||
| Oleaginous yeasts, particularly Yarrowia. lipolytica (Y. lipolytica), are promising platforms for sustainable production of unsaturated fatty acids (UFAs). This study aimed to isolate indigenous oleaginous yeasts from environmental samples in Qom province, Iran, characterize them through phenotypic and molecular analyses, optimize cultivation conditions, and evaluate the impact of optimization on fatty acid profiles. Thirty samples (agricultural soil, rhizosphere, and fruit peels) were collected. Forty-seven yeast isolates were obtained, of which 18 (38.3%) showed lipid accumulation by Sudan Black B staining. ITS sequencing and phylogenetic analysis identified the seven highest lipid producers as Y. lipolytica (99–100% similarity), forming two distinct sub-clusters. One-factor-at-a-time (OFAT) optimization identified optimal conditions as glycerol (10% v/v), NH₄Cl, C/N ratio 60:1, 28°C, pH 5.5, and 96 h, yielding up to 42.3% lipid content (dry cell weight). Comparative GC-MS analysis demonstrated that cultivation optimization induced strain-dependent remodeling of fatty acid profiles. Under identical cultivation conditions, strain QY-1 shifted toward increased polyunsaturated fatty acid production, whereas strain QY-3 preferentially enhanced oleic acid accumulation. These findings highlight the importance of strain-specific physiological responses in tailoring lipid composition through cultivation optimization. This study highlights the value of indigenous Y. lipolytica strains and demonstrates that cultivation optimization can tailor fatty acid profiles for targeted industrial and biomedical applications. | ||
| کلیدواژهها | ||
| Yarrowia lipolytica؛ Oleaginous yeasts؛ Unsaturated fatty acids؛ Phylogenetic analysis؛ ITS sequencing؛ OFAT optimization؛ GC-MS؛ Indigenous isolates؛ Linoleic acid | ||
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آمار تعداد مشاهده مقاله: 3 |
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